
In this white paper you will learn about an intact mass approach using the Intact workflow in Byos® that facilitates the identification, assessment, and resolution of chain shuffling in bispecific antibodies, as well as the manual validation of complex data sets using enhanced visualization tools.
This Application Note describes an intact mass approach to investigate
chain shuffling in bispecific antibodies.The Intact workflow in Byos® provides a streamlined method for
automatic identification and assessment of bispecific antibody structure.The parsimonious charge deconvolution algorithm is well suited to
resolving highly heterogeneous mixtures that result from the undesired
matching of the antibody chains.Enhanced visualization tools facilitate manual inspection and validation of
complex intact data sets.