RNA therapies come in various forms, acting to either up-regulate or down-regulate protein translation to solve disease conditions. Successful development of RNA-based therapeutic candidates requires the interplay of chemistry and biology. Chemical modifications are crucial to ensure efficacy and to protect the therapies from rapid degradation. Discovery project teams are cross-functional, drawing on the skills of chemists and biologists alike. Data management systems for RNA therapies research must also provide cross-functional capabilities and be adept at handling the complex science involved.
RNA therapies are at the leading edge of scientific innovation, driving the latest advancements in chemical modification of biological sequences. Cross-functional teams of biologists and chemists need to search to, and view, a single source of scientific truth but through distinct lenses that match their scientific understanding. Chemists will mostly perform structure searches, biologists will use sequence searches. Either could represent a functionally equivalent query, and therefore find the same results.
RNA therapies research is extending scientific boundaries at a rapid rate. It’s critical that you can fully and accurately define your candidate therapeutics, so you know exactly what you’re working with, and you can establish intellectual property protections.
In order to be able to view your data, you must first capture it. And not just capture in any way. It must be captured in a way that meets the joint goals of not getting in the way of science, but also ensures the data is of the highest quality and accuracy, and can be found again by anyone who needs to.
RNA therapy development requires close coordination between chemists and biologists. Understanding how chemical modifications affect the structure and mode-of-action of RNA molecules is critical to developing effective candidates. It’s crucial that data management systems inherently understand both chemistry and biology, that they store a single representation of therapeutic candidates. To find that data, users must be able to query in their natural “scientific language”–structures for chemists, and sequences for biologists, and and view the results accordingly. This allows cross-functional teams to communicate and collaborate effectively, on high quality data. The Dotmatics Platform, along with its end-user modules, is the only enterprise-scale informatics system that can meet these requirements. Whether you are a start-up, or a multinational biopharma organization, you still need enterprise-scale tools to achieve your corporate objectives.
A critical component of the Dotmatics solution for RNA Therapies development is Bioregister, our biological registration system that generates a full chemical representation of sequence-based entities, using InChI keys. Users can easily transition between sequence and molecular views, or simply use whatever makes most sense to them, as a chemist or a biologist. The full chemical representation is used for uniqueness checking, and chemistry-based searching can be performed. Bioregister also generates HELM notations for these entities, which is often useful for communicating with strategic partners. Bioregister can create implicit bioconjugation linkages based on “click chemistry”, to streamline the definition and capture of conjugated RNAs. Collectively, these capabilities ensure that you know exactly what your candidates are, and you can fully protect your intellectual property (IP).
The Dotmatics solution for RNA therapy development provides the scientific rigor needed to completely capture the full detail of these entities and delivers the broad set of informatics capabilities to fully support your project goals. Experiment templates in the Dotmatics ELN, Studies Notebook, are configurable and can range from simple, “paper-on-glass” protocols used for exploratory experiment, through to high-structured protocols that follow formalized procedures. Studies Notebook templates can be configured to support chemistry or biology-based workflows. In all cases, data are captured, indexed and made available to authorized personnel as soon as possible. This is a hallmark of Dotmatics — to ensure data are findable and usable, not locked away in silos.
Vortex, our advanced analysis and visualization application includes advanced algorithms for chemistry and biology analyses, and can display chemically modified therapeutics in either molecular or sequence format. And ultimately, Dotmatics Browser is the key tool allowing scientists of all types to search, browse and view their data, and make well-informed decisions that accelerate their research and innovation.
Dotmatics Browser is a powerful web-based search and reporting solution that provides federated searching across Dotmatics and third party research databases, enabling research scientists to access all of their data whenever they need it, irrespective of where it is stored.
Dotmatics’ Bioregister is a web-based application for registering sequence-based, chemically-modified and structureless biological entities, allowing biologics discovery organizations to ensure entity uniqueness and protect their intellectual property. Bioregister supports management of a broad set of biologics entities, including DNA, RNA, peptides and proteins, antibodies, conjugates, non-natural peptides and nucleotides, plasmids, cell lines and user-defined entities. It also enables users to record batches and samples for these entities, purification and expression information, and other protein production data.
Dotmatics Studies Notebook is an Electronic Laboratory Notebook (ELN) with flexible templating to capture, store and search experiments from all scientific disciplines.
Dotmatics Vortex is an interactive data visualization and analysis solution for scientific decision support. Building on and extending the spreadsheet paradigm, it provides the data manipulation, statistical analysis and sophisticated plotting capabilities required to explore and understand any complexity and size of data. Vortex is also scientifically aware providing native cheminformatics and bioinformatics analysis and visualizations.
Dotmatics Studies is a highly flexible, innovative screening data management solution to capture and analyze all types of screening experiments from high content (HCS) and high throughput (HTS) to dose-response and DMPK studies.
The Dotmatics Platform is the common framework that supports the Dotmatics web-based scientific applications and allows them to work together to provide users seamless end-to-end workflows. The Platform supports managed deployments in the Cloud or self-supported deployments on premise. An open architecture enables workflows to include third-party software integration. When used together with Dotmatics Browser the Platform allows federated data access across corporate and external research data.The Dotmatics Platform is completely unified – it provides common data models, code and architecture to support business workflows that span the capabilities of the individual applications. This is important for enterprise enablement of research organizations, providing ease of maintenance and development. It gives the lowest possible total cost of ownership compared to platforms that support their applications with less tightly integrated collections of multiple technologies and/or architectures with multiple development histories.
Dotmatics Inventory is a fully searchable sample and materials inventory that tracks chemicals, biologics, instruments and associated data across a hierarchy of locations and manages the dispensing and plating workflows, as samples are moved through an R&D process.
Dotmatics Cascade manages the requesting and assignment of laboratory tasks, and tracking the progression of samples, across multiple teams within an organization or across a collaborating network.